Helicobacter pylori SS1幽门螺杆菌HpSS1 BioVector NTCC质粒载体菌种细胞基因保藏中心

Helicobacter pylori SS1幽门螺杆菌HpSS1 BioVector NTCC质粒载体菌种细胞基因保藏中心 Helicobacter pylori SS1
Cat No.: NTCC502694
Type strain. Quality control strain for API products. CLSI control strain for susceptibility testing.
Medium
Tryptic Soy Agar/Broth (Soybean-Casein Digest Medium, USP)
Trypticase soy agar/broth with defibrinated sheep blood
Biovector NTCC, Inc
No.19,Xizhimen,Beijing,China
http://www.Biovector.net
Tel:010-53513060. 400-800-2947
Growth Conditions
Temperature: 37°C
Atmosphere: Microaerophilic, 3~5% O2 10% CO2
Propagation Procedure
1. This organism is shipped frozen in dry ice and should be stored in vapor phase liquid nitrogen until
ready for use. Just prior to use, thaw vial in water bath at approximately 37°C with gentle agitation
until no ice remains. When thawed, a drop of the suspension may be used to do an immediate wet
mount to observe the unique morphology of this organism and verify its viability by checking for
motility.
2. Aseptically transfer the entire thawed suspension into a fresh #18 broth (35
mL). Mix well. This
suspension can now be used to inoculate agar slant(s), plate(s), or the preferred biphasic culture.
Two #260 plates should be inoculated, one for microaerophilic growth and the second for aerobic
growth. No growth should occur on the plate incubated aerobically.
3. To obtain a biphasic culture, add 0.6 mL of the suspension to a #260 slant. The resulting pool at the
bottom of the slant is where the best, most rapid growth will occur.
4. Incubate at 37°C under microaerophilic conditions using an anaerobe jar with an active catalyst and a
microaerophilic gas generator pack, or other acceptable method, to obtain microaerophilic conditions.
Incubate tubes with caps loose.
5. Within 3 days, good growth should be obtained in the broth pool at the bottom of the slant. Additional
incubation may be required for colonies to appear on the agar plate. Further subcultures can be made
using the broth pool as the inoculum source. Subcultures to biphasic cultures will require only 24 to
48 hours of incubation for good growth.
BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
电话：+86-010-53513060
网址：www.biovector.net [Supplier来源] http://www.biovector.net