639-V cells细胞DSMZ ACC 413 BioVector NTCC质粒载体菌种细胞基因保藏中心

639-V cells细胞DSMZ ACC 413 BioVector NTCC质粒载体菌种细胞基因保藏中心 Cell line: 639-V DSMZ no.: ACC 413Species: human (Homo sapiens)Cell type: ureter transitional cell carcinoma Origin: established from the transitional cell carcinoma (small, grade III) removed from the left ureter of a 69-year-old white man in 1973 Reference(s): 14506, 14505Biosafety level: 1 Permissions and restrictions: A DSMZ Cell Culture Data: Morphology:epithelial-like cells growing adherently in monolayers; imageMedium: 90% Dulbecco's MEM + 10% h.i. FBS Subculture: seed out at ca. 0.5 x 106 cells/25 cm2; split confluent culture 1:5 to 1:10 every 3-4 days using trypsin/EDTA Incubation: at 37 °C with 10% CO2 Doubling time: ca. 30 hours Harvest: cell harvest of ca. 6 x 106 cells/25 cm2 Storage: frozen with 70% medium, 20% FBS, 10% DMSO DSMZ Scientific Data: Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM -, GFAP -, neurofilament -, vimentin + Fingerprint: multiplex PCR of minisatellite markers revealed a unique DNA profile Species: confirmed as human with IEF of AST, MDH Cytogenetics: human hyperdiploid karyotype with 24% polyploidy - 55(50-59)<2n>XX, +1, +3, +5, +6, +8, +11, +12, +14, -15, +20, -21, add(1)(p?21), i(5p), del(6)(q13-14), i(7p), add(8)(p2?), add(11)(p11), del(12)(q21q24), add(18)(q23) - additional copies of 6q-, i(7p), 8p+, der(18) markers present in sidelines together with del(7)(p13-14) Viruses: ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -
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