pTZ57R/T BioVector NTCC质粒载体菌种细胞基因保藏中心

pTZ57R/T BioVector NTCC质粒载体菌种细胞基因保藏中心 pTZ57R/T克隆载体，PCR产物高效TA克隆，低背景，配合细菌转化试剂盒使用可将连接与化学转化同步一小时内完成，可用于T7 RNA聚合酶体外转录，插入基因可轻松采用多个酶切位点酶切。
Linearized pTZ57R vector with 3'-ddT overhangs for TA cloning of PCR products with blue/white screening.
The high quality TA cloning vector
pTZ57R/T is ready to use for efficient ligation with PCR products providing high cloning yields
and low background. To increase the speed, convenience and efficiency of cloning, the
InsTAclone PCR Cloning Kit has been combined with the TransformAid Bacterial
Transformation Kit – a set of solutions for preparation of chemically competent E. coli cells.
According to our protocol, ligation and preparation of competent cells is performed in parallel.
Therefore, it only takes approximately one hour from the completion of PCR to plating of
transformed cells. Our transformation protocol is often faster than transformation of
commercially available competent cells.
The DNA insert can be readily excised from the versatile polylinker of pTZ57R/T, sequenced
using standard M13/pUC primers or in vitro transcribed with T7 RNA polymerase.
CLONING PRINCIPLE
The InsTAclone PCR Cloning Kit takes advantage of the terminal transferase activity of
Taq DNA polymerase and other non-proofreading thermostable DNA polymerases. Such
enzymes add a single 3’-A overhang to both ends of the PCR product. The structure of these
PCR products favors direct cloning into a linearized cloning vector with single 3’-ddT overhangs.
Such overhangs at the vector cloning site not only facilitate cloning, but also prevent the
recircularization of the vector. As a result, more than 90% of recombinant clones contain the
vector with an insert. Recombinant clones are selected based on blue/white screening.
BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
电话：+86-010-53513060
网址：www.biovector.net [Supplier来源] http://www.biovector.net