Bacteroides vulgatus普通拟杆菌菌株 BioVector NTCC质粒载体菌种细胞基因保藏中心

Bacteroides vulgatus普通拟杆菌菌株 BioVector NTCC质粒载体菌种细胞基因保藏中心 Bacteroides vulgatus
Cat No.: NTCC8482

Strain Designations [NCTC 11154]
Application Media testing
Quality control strain
Sterility testing
Pharmaceutical and Personal Care
Biosafety Level 2
Product Format freeze-dried
Genome Sequenced Strain Bacteroides vulgatus ATCC 8482, complete genome
Type Strain yes
Comments Genome sequenced strain
This organism is included in the bioMerieux VITEK 2 ANC Comprehensive and Traditional Quality Control Organism Sets
References Moore WE, Holdeman LV. New names and combinations in the genera Bacteroides Castellani and Chalmers, Fusobacterium Knorr, Eubacterium Prevot, Propionibacterium Delwich, and Lactobacillus Orla-Jensen. Int. J. Syst. Bacteriol. 23: 69-74, 1973.

Medium
Medium 2107: Modified Reinforced Clostridial Agar/Broth Medium (pre-reduced)
Medium 260: Trypticase soy agar/broth with defibrinated sheep blood

Growth Conditions
Temperature: 37°C
Atmosphere: Anaerobic gas mixture, 80% N2-10% CO2-10% H2

Medium: 2107 Modified Reinforced Clostridial Agar/Broth
(pre-reduced)
Agar Medium
Reinforced Clostridial Medium (BD 218081)…….…..38 g
Agar………………………………………………………14.5 g
DI Water………………………………………………….1000 ml
Combine ingredients and boil to dissolve agar. Dispense and autoclave at 121ºC.
If making plates, autoclave at 121ºC, let cool to 55ºC and dispense.
Broth Medium
Peptone……………………………………..10.0 g
Beef Extract…………………………………10.0 g
Yeast Extract………………………………..3.0 g
Dextrose…………………………………….5.0 g
NaCl…………………………………………5.0 g
Soluble Starch……………………………...1.0 g
L-Cysteine HCl……………………………..0.5 g
Sodium Acetate…………………………….3.0 g
Resazurin (0.025%)………………………..4 ml
DI Water……………………………………..1000 ml
Combine ingredients and dissolve. Adjust pH to 6.8. Dispense and autoclave at
121ºC.
*To pre-reduce broth: Add all ingredients except L-Cysteine HCl. Bring medium
to boil to drive off oxygen. Cool down medium while bubbling with oxygen free
gas. Add L-Cysteine HCl and adjust pH to 6.8. Dispense under same gas phase
and autoclave at 121C.
Oxoid’s Lab-Lemco Powder (LP0029) corresponds to BD’s Beef Extract except it
gives a much lighter color to the medium. It may be substituted for Beef Extract if
desired.
BD and Oxoid Reinforced Clostridial formulations contain agar. This Modification
is agar-free.
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Medium: 260 Tryptic Soy Agar/Broth with 5% Sheep Blood
(defibrinated)
Agar Medium
Tryptic Soy Agar (BD 236950)……………40 g
Sheep Blood (defibrinated)………………..50 ml
DI Water……………………………………...950 ml
Autoclave at 121ºC. Cool sterilized medium to ~47ºC. Aseptically add 50 ml of
room temperature defibrinated sheep blood. Gently mix and dispense as
required.
Broth Medium
Tryptic Soy Broth (BD 211825)…………….30.0 g
Sheep Blood (defibrinated)…………………50 ml
DI Water…………………………………..…..950 ml
Follow same procedure as agar medium above.
If commercialized product is not available, Tryptic Soy Agar can be made from
scratch, by following the formulation below:
Tryptic Soy Agar Composition
Tryptone…………………………15.0 g
Soytone………………………….5.0 g
NaCl……………………………...5.0 g
Agar……………………………...15.0 g
DI Water………………………….950 ml
pH to 7.3 +/- 0.2. (Tryptone-Pancreatic Digest of Casein; Soytone-Papaic Digest
of Soybean Meal). To make Tryptic Soy Broth from scratch, follow Tryptic Soy
Agar formulation but omit agar.

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