IPEC-J2 Cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

IPEC-J2 Cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心 IPEC-J2 Cells
Cat No.: NTCC510701

Cell line:
IPEC-J2
Species:
porcine (pig) (Sus scrofa)
Cell type:
intestine (jejunum)
Origin:
established from normal intestinal epithelium cells isolated from the jejunum of a neonatal, unsuckled pig
Biosafety level:
1
Permissions and restrictions:
A

Cell Culture Data:
Morphology:
epitheloid cells growing adherently as monolayer;


Medium:
90% Dulbecco´s MEM (4.5 g/L glucose) + 10% h.i. FBS
Subculture:
seed out at ca. 1-2 x 106 cells/80 cm2 flask; split confluent culture 1:2 to 1:3 every 3-4 days using trypsin/EDTA
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 40-60 hours
Harvest:
cell harvest of ca. 8 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO

Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Species:
confirmed as pig by species PCR

Complete growth medium, 95%; DMSO, 5%




Seed out at ca. 1-2 x 106 cells/80 cm2 flask; split confluent culture 1:2 to 1:3 every 3-4 days using trypsin/EDTA




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