pGreenIIM DR5v2-ntdTomato/DR5-n3GFP BioVector NTCC质粒载体菌种细胞基因保藏中心

pGreenIIM DR5v2-ntdTomato/DR5-n3GFP BioVector NTCC质粒载体菌种细胞基因保藏中心 Expresses nuclear tdTomato from auxin-dependent DR5v2 (TGTCGG) promoter and nuclear 3xGFP from the DR5 (TGTCTC) promoter
Please note that this construct contains GFPmut2 NOT EGFP as indicated in the full sequence. This does not have any functional consequences for the plasmid. The diagnostic digest indicates this plasmid may be bigger than the assembled sequence suggests.
Vector backbone pGreenII
(Search Vector Database)
Backbone size w/o insert (bp) 2580
Total vector size (bp) 9692
Vector type Plant Expression
Selectable markers Methothrexate
GROWTH IN BACTERIA

Bacterial Resistance(s) Kanamycin
Growth Temperature 37°C
Growth Strain(s) DH5alpha
Copy number Low Copy
GENE/INSERT 1

Gene/Insert name ntdTomato
Species Synthetic
Insert Size (bp) 1518
GenBank IDKJ081243.1
Promoter Auxin-dependent DR5v2 promoter containing 9 reverse TGTCGG sites and -46 35S promoter
CLONING INFORMATION FOR GENE/INSERT 1

Cloning method Restriction Enzyme
5′ cloning site HindIII (unknown if destroyed)
3′ cloning site XhoI (unknown if destroyed)
5′ sequencing primer M13_pUC_fwd
(Common Sequencing Primers)
GENE/INSERT 2

Gene/Insert name n3xGFPmut2
Species Synthetic
Insert Size (bp) 2100
Mutation 3 copies of GFPmut2 with N-terminal SV40 NLS
GenBank IDAF302837
Promoter Auxin-dependent DR5 promoter containing 9 reverse TGTCTC sites and -46 35S promoter
CLONING INFORMATION FOR GENE/INSERT 2

Cloning method Restriction Enzyme
5′ cloning site BamHI (unknown if destroyed)
3′ cloning site XbaI (unknown if destroyed)
5′ sequencing primer dsRed1_C_primer
BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
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