pMBP-MS2 BioVector NTCC质粒载体菌种细胞基因保藏中心

pMBP-MS2 BioVector NTCC质粒载体菌种细胞基因保藏中心 can be used to isolate RNA binding complexes
Vector backbone pMal-c
(Search Vector Database)
Backbone manufacturer NEB
Total vector size (bp) 6442
Vector type Bacterial Expression
GROWTH IN BACTERIA

Bacterial Resistance(s) Ampicillin
Growth Temperature 37°C
Growth Strain(s) XL-2 Blue
Growth instructions Cannot be grown in DH5 alpha. Has to be grown in a strain that requires IPTG induction of the promoter, as E. coli don't like it if it's constitutively expressed. TB1 strain is also good.
Copy number Unknown
GENE/INSERT

Gene/Insert name MS2
Species Synthetic
Mutation double mutation (V75Q and A81G) that prevents oligomerization
Promoter tac
Tag / Fusion Protein
MBP (N terminal on insert)
CLONING INFORMATION

Cloning method Restriction Enzyme
5′ cloning site StuI (destroyed during cloning)
3′ cloning site HindIII (not destroyed)
5′ sequencing primer M13pUC-Rev or MBP-F
3′ sequencing primer M13pUC-Fwd
BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
电话：+86-010-53513060
网址：www.biovector.net [Supplier来源] http://www.biovector.net