HCT116 DKO cells细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

HCT116 DKO（knockouts of both DNA methyltransferases DNMT1 (-/-) and DNMT3b (-/-)）

HCT116 DKO (double knock-out) cell line contains genetic knockouts of both DNA methyltransferases DNMT1 (-/-) and DNMT3b (-/-). The DNA derived from HCT116 DKO cells has a low level of DNA methylation and therefore can be used as a negative control for DNA methylation analysis.
HCT116 double knockout (DKO) cells exhibit lower IL-8 secretion and reduced monocyte chemotaxis than parental cells. HCT116 DKO cells have lower cFLIP expression and higher caspase activity than parental cells. lower NF-kapaB activity than parental cells.


Figure 1. An assay for complete methylation by M.SssI methytransferase. Digestion of non-methylated and methylated HCT116 DKO DNA with restriction enzymes MspI and HpaII. MspI digests both nonmethylated and methylated DNA. HpaII is sensitive to CpG methylation.

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