MLE-12 cells细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

MLE-12 cells细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心 MLE 12 cell line Cat No.: NTCC597128 Organism Mus musculus, transgenic, mouse, transgenic Tissue lung Cell Type epithelial; SV40 transformed Product Format frozen Morphology epithelial Culture Properties adherent Biosafety Level 2 [Cells contain Papovavirus] Age 5 month old Gender female Strain FVB/N Derivation This line was established in 1992 by Kathryn A. Wikenheiser from pulmonary tumors in a mouse transgenic for the SV40 large T antigen under the control of the promoter region of the human surfactant protein C gene. Clinical Data female 5 months Genes Expressed lung surfactant proteins B and C (SP-B, SP-C) Tumorigenic Yes Effects Yes, tumors were generated within 6 to 9 months in nude mice Comments The cells express the mRNA for large T antigen. Lung surfactant proteins B and C were detected. The cells secrete phospholipids in response to phorbol esters and ATP but not in response to forskolin. Complete Growth Medium HITES medium supplemented with 2% fetal bovine serum Subculturing Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes. 1. Remove and discard culture medium. 2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. 3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. 4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. 5. Add appropriate aliquots of the cell suspension to new culture vessels. 6. Incubate cultures at 37°C. Subcultivation Ratio: A subcultivation ratio of 1:5 to 1:10 is recommended Medium Renewal: Twice per week Cryopreservation Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase Culture Conditions Atmosphere: air, 95%; carbon dioxide (CO2), 5% Population Doubling Time 7 to 9 hrs Deposited As mouse, transgenic Year of Origin 1992 References Wikenheiser KA, et al. Simian virus 40 large T antigen directed by transcriptional elements of the human surfactant protein C gene produces pulmonary adenocarcinomas in transgenic mice. Cancer Res. 52: 5342-5352, 1992. PubMed: 1394139
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