IEC-6 cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

IEC-6 cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心 IEC-6 cell line
Cat No.: NTCC595981

Organism Rattus norvegicus, rat
Tissue small intestine/epithelium
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease normal
Gender male
Applications This cell line is a suitable transfection host.
Clinical Data male
Comments Cell growth is inhibited by cortisol.
The cells express antigens characteristic of intestinal epithelia.
The depositor notes that the cells maintain a constant growth rate and cell morphology until approximately the 20th passage, thereafter, either cell growth rapidly declines or morphological changes are observed.
Complete Growth Medium Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 0.1 Unit/ml bovine insulin, 90%; fetal bovine serum, 10%
Subculturing Remove medium, and rinse the monolayer with fresh 0.25% trypsin, 0.53 mM EDTA solution. Remove the trypsin, add fresh trypsin and let the culture sit at room temperature (or at 37°C) until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Twice per week
Cryopreservation Freeze medium: culture medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions Temperature: 37°C
Passage History The depositor notes that the cells maintain a constant growth rate and cell morphology until approximately the 20th passage, thereafter, either cell growth rapidly declines or morphological changes are observed.
References Quaroni A, et al. Fibronectin synthesis by epithelial crypt cells of rat small intestine. Proc. Natl. Acad. Sci. USA 75: 5548-5552, 1978. PubMed: 103096

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