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BPK1520 BioVector NTCC质粒载体菌种细胞基因保藏中心 Human expression plasmid for SpCas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sp-sgRNA
Use BsmBI sites to insert sgRNA spacer sequence into the vector.
Vector backbone MLM3636
(Search Vector Database)
Backbone manufacturer Joung lab (Addgene BioVector-0 43860)
Vector type Mammalian Expression, CRISPR
GROWTH IN BACTERIA
Bacterial Resistance(s) Ampicillin
Growth Temperature 37°C
Growth Strain(s) XL1 Blue
Copy number High Copy
GENE/INSERT
Gene/Insert name SpCas9 gRNA backbone, without spacer sequence
Species Synthetic
Promoter U6
CLONING INFORMATION
Cloning method Gibson Cloning
5′ sequencing primer OS280 (5'-CAGGGTTATTGTCTCATGAGCGG-3')
BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
电话：+86-010-53513060
网址：www.biovector.net [Supplier来源] http://www.biovector.net
Use BsmBI sites to insert sgRNA spacer sequence into the vector.
Vector backbone MLM3636
(Search Vector Database)
Backbone manufacturer Joung lab (Addgene BioVector-0 43860)
Vector type Mammalian Expression, CRISPR
GROWTH IN BACTERIA
Bacterial Resistance(s) Ampicillin
Growth Temperature 37°C
Growth Strain(s) XL1 Blue
Copy number High Copy
GENE/INSERT
Gene/Insert name SpCas9 gRNA backbone, without spacer sequence
Species Synthetic
Promoter U6
CLONING INFORMATION
Cloning method Gibson Cloning
5′ sequencing primer OS280 (5'-CAGGGTTATTGTCTCATGAGCGG-3')
BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
电话：+86-010-53513060
网址：www.biovector.net [Supplier来源] http://www.biovector.net
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