AK2 (V617F)-pcw107-V5 BioVector NTCC质粒载体菌种细胞基因保藏中心

AK2 (V617F)-pcw107-V5 BioVector NTCC质粒载体菌种细胞基因保藏中心 when used to produce lentivirus, express physiological levels of insert
Vector backbone pcw107 (V5)
(Search Vector Database)
Backbone manufacturer John Doench/Kathleen Ottina
Backbone size w/o insert (bp) 9240
Vector type Lentiviral
Selectable markers Puromycin
GROWTH IN BACTERIA

Bacterial Resistance(s) Ampicillin
Growth Temperature 37°C
Growth Strain(s) Stbl3
Copy number Unknown
GENE/INSERT

Gene/Insert name JAK2
Alt name NM_004972.3
Species H. sapiens (human)
Insert Size (bp) 3399
Mutation V617F
Entrez Gene JAK2 (a.k.a. JTK10, THCYT3)
Promoter PGK
Tag / Fusion Protein
V5 (C terminal on backbone)
CLONING INFORMATION

Cloning method Gateway Cloning
5′ sequencing primer hPGK-F
3′ sequencing primer WPRE-R
(Common Sequencing Primers)
RESOURCE INFORMATION

Supplemental Documents
pCW107 Destseq-V5
A portion of this plasmid was derived from a plasmid made by William Vainchecker Lab, Institut Gustave Roussy
Terms and Licenses
UBMTA
DEPOSITOR COMMENTS

TGGT (N-term barcode, 17 nt upstream of CDS start). Working name: CTK-D1-V5
BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
电话：+86-010-53513060
网址：www.biovector.net [Supplier来源] http://www.biovector.net