pDONR P4-P1R-EGFP BioVector NTCC质粒载体菌种细胞基因保藏中心

pDONR P4-P1R-EGFP BioVector NTCC质粒载体菌种细胞基因保藏中心 Contributes the coding sequence for EGFP as the 5’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.
Vector backbone pDONRP4-P1R
(Search Vector Database)
Backbone manufacturer Life Technologies/Invitrogen
Backbone size w/o insert (bp) 2655
Total vector size (bp) 3363
Vector type Gateway entry vector
GROWTH IN BACTERIA

Bacterial Resistance(s) Kanamycin
Growth Temperature 37°C
Growth Strain(s) Top10
Copy number High Copy
GENE/INSERT

Gene/Insert name EGFP
Species Synthetic; Aequorea victoria
Insert Size (bp) 708
Mutation Contains a Kozak sequence. Does not contain a stop codon. Aminoacid 40 is valine in our sequence, while it is phenylalanine in other EGFP sequences. This does not seem to affect fluorescence.
Promoter none
CLONING INFORMATION

Cloning method Gateway Cloning
5′ sequencing primer M13 forward (-20)
3′ sequencing primer M13 reverse
BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
电话：+86-010-53513060
网址：www.biovector.net [Supplier来源] http://www.biovector.net