MDA-MB-468 cells细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

MDA-MB-468 cells细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心 Organism Homo sapiens, human
Tissue mammary gland/breast; derived from metastatic site: pleural effusion
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1.
Disease adenocarcinoma
Age 51 years
Gender female
Ethnicity Black
Applications This cell line is a suitable transfection host.
Karyotype modal number = 64; range = 60 to 67.
The cell line is aneuploid human, presumably female (X, abnormal X) with most chromosome counts in the hypotriploid range.; Normal chromosomes X, N2, N3, N7, N8, N10, and N22 are clearly under-represented due to their involvement in the formation of the many marker (19) chromosomes present in this cell line.; A normal chromosome N1 (or two) is identified in each karyotype, but, in addition, regions of chromosome N1 are also present in five different marker chromosomes.; Variation is evident in the normal and marker chromosome copy number from karyotype to karyotype.
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Derivation The MDA-MB-468 cell line was isolated in 1977 by R. Cailleau, et al., from a pleural effusion of a 51-year-old Black female patient with metastatic adenocarcinoma of the breast.
Clinical Data 51 years
Although the tissue donor was heterozygous for the G6PD alleles, the cell line consistently showed only the G6PD A phenotype.
Black
female

Antigen Expression Blood Type AB; HLA Aw23, Aw30, B27, Bw35, Cw2, Cw4 (patient)
Receptor Expression epidermal growth factor (EGF); transforming growth factor alpha (TGF alpha)
Tumorigenic Yes
Effects Yes, in nude mice inoculated subcutaneously with 10(7) cells.
(Tumors developed within 21 days at 100% frequency (5/5).)
Comments Although the tissue donor was heterozygous for the G6PD alleles, the cell line consistently showed only the G6PD A phenotype. There is a G -> A mutation in codon 273 of the p53 gene resulting in an Arg -> His substitution. EGF receptor is present at 1 X 106per cell.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
(Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation)


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