OCI-LY3细胞株ACC 761 BioVector NTCC质粒载体菌种细胞基因保藏中心

OCI-LY3细胞株ACC 761 BioVector NTCC质粒载体菌种细胞基因保藏中心 Cell line:
OCI-LY3
NTCC no.:
ACC 761
Species:
human (Homo sapiens)
Cell type:
B cell lymphoma
Origin:
established in 1983 from the bone marrow sample of a 52-year-old man with B-cell non-Hodgkin lymphoma (B-NHL, diffuse large B-cell lymphoma, DLBCL, stage 4B) at relapse; cells were described in the literature to be EBV-negative and lacking a classical t(8;14) translocation
Reference(s):
14427, 18975, 15976
Biosafety level:
1
Permissions and restrictions:
A

NTCC Cell Culture Data:
Morphology:
round cells growing singly or in clumps in suspension; image;

Medium:
80% RPMI 1640 + 20% h.i. FBS
Subculture:
seed out at ca. 0.2-0.3 x 106 cells/ml; optimal split ratio 1:3 - 1:5 every 2-3 days; after thawing cell viability may be as low as 50%, but cells should recover quickly within a week; maintain at ca. 0.1-0.8 x 106 cells/ml
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 24 hours
Harvest:
maximum density at ca. 0.9 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO

NTCC Scientific Data:
Mycoplasma:
negative in PCR assay
Immunology:
CD3 -, CD10 -, CD13 -, CD19 -, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 +, CD138 -, HLA-DR +, sm/cyIgG +, sm/cyIgM -, sm/cykappa -, sm/cylambda +; in the referenced paper this cell line is described as CD19 +, cykappa +; image
Fingerprint:
genetic typing showed a pattern of short tandem repeats (STR) matching the reference profile of the international STR database
Cytogenetics:
human flat-moded hypertriploid karyotype; 72-77<3n>XXYY, +1, +9, -10, +13, +14, -17, +19, +20, +22, der(1)t(1;17)(p13;q12)x2, der(4)t(4;18)(q31;q21)x2, del(6)(q13)x2, der(6)t(6;6)(p24;q12), der(7)t(6;7)(p24;p22), der(14)t(14;19)(q32;q13.3)x2, del(18)(q21), der(19)t(4;19)(q21;q13)t(4;18)(q31;q21)x2, der(19)t(14;19), dup(20)(q11q13)x2; sdl with der(6)t(6;12)(p21;q21), der(7)t(5;7)(?p15;p24) etc; resembles published karyotypes; carries cryptic t(14;19) with rearrangement of IGH and SPIB, and t(4;18) with copy number amplification of the BCL2 region
Viruses:
PCR: HBV -, HCV -, HIV -, MLV -
[Supplier来源] http://www.biovector.net