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pfu高保真聚合酶高表达质粒载体菌种-BioVector NTCC保藏中心

  • 价  格:¥6920
  • 货  号:pfu DNA polymerase expression plasmid
  • 产  地:北京
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pfu DNA polymerase聚合酶高表达质粒载体菌种

DescriptionBack to Top

High-Fidelity DNA Polymerases are important for applications in which the DNA sequence needs to be correct after amplification. Manufactured and quality-controlled at New England Biolabs, Thermo Scientific® Phusion High-Fidelity DNA Polymerase offers both high fidelity and robust performance, and thus can be used for all PCR applications. Its unique structure, a novel Pyrococcus-like enzyme fused with a processivity-enhancing domain, increases fidelity and speed. Phusion DNA Polymerase is an ideal choice for cloning and can be used for long or difficult amplicons. With an error rate >50-fold lower than that of Taq DNA Polymerase and 6-fold lower than that of Pyrococcus furiosus DNA Polymerase (1), Phusion is one of the most accurate thermostable polymerase available. Phusion DNA Polymerase possesses 5´→ 3´ polymerase activity, 3´→ 5´ exonuclease activity and will generate blunt-ended products.

Phusion High-Fidelity PCR Master Mix with HF Buffer is a 2X master mix consisting of Phusion DNA Polymerase, deoxynucleotides and reaction buffer that has been optimized and includes MgCl2. All that is required is the addition of template, primers and water.

Fidelity assays were performed using a lacI-based method modified from Frey & Suppman, 1995.
A 3.8 kb fragment was amplified from 50 ng of Jurkat gDNA using different polymerases. Reactions were carried out according to the manufacturer's recommended conditions. Extension times are indicated (in minutes). Ladder L is a 1 kb DNA Ladder.

Product Source

An E. coli strain that carries the Phusion DNA Polymerase gene.

Reagents Supplied

The following reagents are supplied with this product:


Store at (°C)Concentration
DMSO
100%

Advantages and FeaturesBack to Top

Features

  • Robust Reactions - Maximal success with minimal optimization.

  • Extreme Fidelity - > 50x greater than Taq

  • High Speed - Dramatically reduced extension times (10x faster than Pfu)

  • High Yield - Increased product yield using minimal amount of enzyme.

  • Versatile - Can be used for routine per as well as long or difficult templates.

Applications

  • PCR

  • Cloning

  • Long or Difficult Amplification

  • High-Throughput PCR

Properties and UsageBack to Top

Storage Temperature

-20°C

Heat Inactivation

No

Unit Assay Conditions

25 mM TAPS-HCl (pH 9.3 @ 25°C), 50 mM KCl, 2 mM MgCl2 , 1 mM b-mercaptoethanol, 200 µM dNTPs including [3H]-dTTP and 15 nM primed M13 DNA.

NotesBack to Top

  1. Products specifications for individual components in the Phusion High-Fidelity PCR Master Mix with HF Buffer are available separately.

ReferencesBack to Top

  1. Frey, B. and Suppman, B. (1995). Biochemica. 2, 34-35.

  2. Chester, N. and Marshak, D.R. (1993). Analytical Biochemistry. 209, 284-290.



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