二代慢病毒包装载体pSPAX2质粒-BioVector NTCC保藏中心

LOCUS       psPAX2	10703 bp 	DNA   circular	SYN
DEFINITION  psPAX2
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.biovector.net/
COMMENT     VNTAUTHORNAME|biovector.net|
FEATURES             Location/Qualifiers
     source          1..10703
                     /organism="psPAX2"
                     /mol_type="other DNA"
     misc_feature    84..371
                     /label="CAG_enhancer"
     CDS             255..734
                     /label="ORF frame 3"
     CDS             complement(802..1533)
                     /label="ORF frame 3"
     CDS             complement(843..1346)
                     /label="ORF frame 1"
     misc_feature    1678..1697
                     /label="pCAG_F_primer"
     gene            1780..3282
                     /label="Gag_HIV (variant)"
                     /gene="Gag_HIV (variant)"
     CDS             1780..3282
                     /label="ORF frame 1"
     CDS             3348..6086
                     /label="ORF frame 3"
     misc_feature    5775..5790
                     /label="cPPT"
     CDS             6538..7686
                     /label="ORF frame 1"
     misc_feature    6660..6893
                     /label="RRE"
     misc_feature    7416..7445
                     /label="HIV_Rev_NES"
     terminator      7731..8160
                     /label="rb_glob_PA_terminator"
     promoter        complement(8202..8220)
                     /label="M13_reverse_primer"
     misc_feature    complement(8219..8241)
                     /label="M13_pUC_rev_primer"
     promoter        complement(8255..8284)
                     /label="lac_promoter"
     promoter        8390..8569
                     /label="SV40_promoter"
     rep_origin      8425..8502
                     /label="SV40_origin"
     misc_feature    8487..8506
                     /label="SV40pro_F_primer"
     terminator      8583..8714
                     /label="SV40_PA_terminator"
     misc_feature    8671..8690
                     /label="EBV_rev_primer"
     rep_origin      complement(8938..9557)
                     /label="pBR322_origin"
     gene            complement(9712..10572)
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             complement(9712..10572)
                     /label="ORF frame 3"
     promoter        complement(10614..10642)
                     /label="AmpR_promoter"
ORIGIN
    1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
   61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
  121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
  181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac
  241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
  301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
  361 tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc
  421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
  481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg
  541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
  601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
  661 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc
  721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
  781 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag
  841 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg
  901 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg
  961 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc
 1021 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
 1081 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc
 1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc
 1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
 1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gccccggagc gccggcggct
 1321 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg
 1381 gacttccttt gtcccaaatc tggcggagcc gaaatctggg aggcgccgcc gcaccccctc
 1441 tagcgggcgc gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt
 1501 cgtgcgtcgc cgcgccgccg tccccttctc catctccagc ctcggggctg ccgcaggggg
 1561 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg
 1621 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca
 1681 acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attcgggccg gccgcgttga
 1741 cgcgcacggc aagaggcgag gggcggcgac tggtgagaga tgggtgcgag agcgtcagta
 1801 ttaagcgggg gagaattaga tcgatgggaa aaaattcggt taaggccagg gggaaagaaa
 1861 aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat
 1921 cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc
 1981 cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt
 2041 gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag
 2101 caaaacaaaa gtaagaaaaa agcacagcaa gcagcagctg acacaggaca cagcaatcag
 2161 gtcagccaaa attaccctat agtgcagaac atccaggggc aaatggtaca tcaggccata
 2221 tcacctagaa ctttaaatgc atgggtaaaa gtagtagaag agaaggcttt cagcccagaa
 2281 gtgataccca tgttttcagc attatcagaa ggagccaccc cacaagattt aaacaccatg
 2341 ctaaacacag tggggggaca tcaagcagcc atgcaaatgt taaaagagac catcaatgag
 2401 gaagctgcag aatgggatag agtgcatcca gtgcatgcag ggcctattgc accaggccag
 2461 atgagagaac caaggggaag tgacatagca ggaactacta gtacccttca ggaacaaata
 2521 ggatggatga cacataatcc acctatccca gtaggagaaa tctataaaag atggataatc
 2581 ctgggattaa ataaaatagt aagaatgtat agccctacca gcattctgga cataagacaa
 2641 ggaccaaagg aaccctttag agactatgta gaccgattct ataaaactct aagagccgag
 2701 caagcttcac aagaggtaaa aaattggatg acagaaacct tgttggtcca aaatgcgaac
 2761 ccagattgta agactatttt aaaagcattg ggaccaggag cgacactaga agaaatgatg
 2821 acagcatgtc agggagtggg gggacccggc cataaagcaa gagttttggc tgaagcaatg
 2881 agccaagtaa caaatccagc taccataatg atacagaaag gcaattttag gaaccaaaga
 2941 aagactgtta agtgtttcaa ttgtggcaaa gaagggcaca tagccaaaaa ttgcagggcc
 3001 cctaggaaaa agggctgttg gaaatgtgga aaggaaggac accaaatgaa agattgtact
 3061 gagagacagg ctaatttttt agggaagatc tggccttccc acaagggaag gccagggaat
 3121 tttcttcaga gcagaccaga gccaacagcc ccaccagaag agagcttcag gtttggggaa
 3181 gagacaacaa ctccctctca gaagcaggag ccgatagaca aggaactgta tcctttagct
 3241 tccctcagat cactctttgg cagcgacccc tcgtcacaat aaagataggg gggcaattaa
 3301 aggaagctct attagataca ggagcagatg atacagtatt agaagaaatg aatttgccag
 3361 gaagatggaa accaaaaatg atagggggaa ttggaggttt tatcaaagta ggacagtatg
 3421 atcagatact catagaaatc tgcggacata aagctatagg tacagtatta gtaggaccta
 3481 cacctgtcaa cataattgga agaaatctgt tgactcagat tggctgcact ttaaattttc
 3541 ccattagtcc tattgagact gtaccagtaa aattaaagcc aggaatggat ggcccaaaag
 3601 ttaaacaatg gccattgaca gaagaaaaaa taaaagcatt agtagaaatt tgtacagaaa
 3661 tggaaaagga aggaaaaatt tcaaaaattg ggcctgaaaa tccatacaat actccagtat
 3721 ttgccataaa gaaaaaagac agtactaaat ggagaaaatt agtagatttc agagaactta
 3781 ataagagaac tcaagatttc tgggaagttc aattaggaat accacatcct gcagggttaa
 3841 aacagaaaaa atcagtaaca gtactggatg tgggcgatgc atatttttca gttcccttag
 3901 ataaagactt caggaagtat actgcattta ccatacctag tataaacaat gagacaccag
 3961 ggattagata tcagtacaat gtgcttccac agggatggaa aggatcacca gcaatattcc
 4021 agtgtagcat gacaaaaatc ttagagcctt ttagaaaaca aaatccagac atagtcatct
 4081 atcaatacat ggatgatttg tatgtaggat ctgacttaga aatagggcag catagaacaa
 4141 aaatagagga actgagacaa catctgttga ggtggggatt taccacacca gacaaaaaac
 4201 atcagaaaga acctccattc ctttggatgg gttatgaact ccatcctgat aaatggacag
 4261 tacagcctat agtgctgcca gaaaaggaca gctggactgt caatgacata cagaaattag
 4321 tgggaaaatt gaattgggca agtcagattt atgcagggat taaagtaagg caattatgta
 4381 aacttcttag gggaaccaaa gcactaacag aagtagtacc actaacagaa gaagcagagc
 4441 tagaactggc agaaaacagg gagattctaa aagaaccggt acatggagtg tattatgacc
 4501 catcaaaaga cttaatagca gaaatacaga agcaggggca aggccaatgg acatatcaaa
 4561 tttatcaaga gccatttaaa aatctgaaaa caggaaaata tgcaagaatg aagggtgccc
 4621 acactaatga tgtgaaacaa ttaacagagg cagtacaaaa aatagccaca gaaagcatag
 4681 taatatgggg aaagactcct aaatttaaat tacccataca aaaggaaaca tgggaagcat
 4741 ggtggacaga gtattggcaa gccacctgga ttcctgagtg ggagtttgtc aatacccctc
 4801 ccttagtgaa gttatggtac cagttagaga aagaacccat aataggagca gaaactttct
 4861 atgtagatgg ggcagccaat agggaaacta aattaggaaa agcaggatat gtaactgaca
 4921 gaggaagaca aaaagttgtc cccctaacgg acacaacaaa tcagaagact gagttacaag
 4981 caattcatct agctttgcag gattcgggat tagaagtaaa catagtgaca gactcacaat
 5041 atgcattggg aatcattcaa gcacaaccag ataagagtga atcagagtta gtcagtcaaa
 5101 taatagagca gttaataaaa aaggaaaaag tctacctggc atgggtacca gcacacaaag
 5161 gaattggagg aaatgaacaa gtagatgggt tggtcagtgc tggaatcagg aaagtactat
 5221 ttttagatgg aatagataag gcccaagaag aacatgagaa atatcacagt aattggagag
 5281 caatggctag tgattttaac ctaccacctg tagtagcaaa agaaatagta gccagctgtg
 5341 ataaatgtca gctaaaaggg gaagccatgc atggacaagt agactgtagc ccaggaatat
 5401 ggcagctaga ttgtacacat ttagaaggaa aagttatctt ggtagcagtt catgtagcca
 5461 gtggatatat agaagcagaa gtaattccag cagagacagg gcaagaaaca gcatacttcc
 5521 tcttaaaatt agcaggaaga tggccagtaa aaacagtaca tacagacaat ggcagcaatt
 5581 tcaccagtac tacagttaag gccgcctgtt ggtgggcggg gatcaagcag gaatttggca
 5641 ttccctacaa tccccaaagt caaggagtaa tagaatctat gaataaagaa ttaaagaaaa
 5701 ttataggaca ggtaagagat caggctgaac atcttaagac agcagtacaa atggcagtat
 5761 tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag
 5821 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
 5881 aaaattttcg ggtttattac agggacagca gagatccagt ttggaaagga ccagcaaagc
 5941 tcctctggaa aggtgaaggg gcagtagtaa tacaagataa tagtgacata aaagtagtgc
 6001 caagaagaaa agcaaagatc atcagggatt atggaaaaca gatggcaggt gatgattgtg
 6061 tggcaagtag acaggatgag gattaacaca tggaattctg caacaactgc tgtttatcca
 6121 tttcagaatt gggtgtcgac atagcagaat aggcgttact cgacagagga gagcaagaaa
 6181 tggagccagt agatcctaga ctagagccct ggaagcatcc aggaagtcag cctaaaactg
 6241 cttgtaccaa ttgctattgt aaaaagtgtt gctttcattg ccaagtttgt ttcatgacaa
 6301 aagccttagg catctcctat ggcaggaaga agcggagaca gcgacgaaga gctcatcaga
 6361 acagtcagac tcatcaagct tctctatcaa agcagtaagt agtacatgta atgcaaccta
 6421 taatagtagc aatagtagca ttagtagtag caataataat agcaatagtt gtgtggtcca
 6481 tagtaatcat agaatatagg aaaatggccg ctgatcttca gacctggagg aggagatatg
 6541 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
 6601 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
 6661 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcctcaatg
 6721 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
 6781 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
 6841 ctccaagcaa gaatcctagc tgtggaaaga tacctaaagg atcaacagct cctagggatt
 6901 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
 6961 aataaatctc tggaacagat ctggaatcac acgacctgga tggagtggga cagagaaatt
 7021 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
 7081 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
 7141 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
 7201 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
 7261 tcgtttcaga cccacctccc aatcccgagg ggacccgaca ggcccgaagg aatagaagaa
 7321 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc cttggcactt
 7381 atctgggacg atctgcggag cctgtgcctc ttcagctacc accgcttgag agacttactc
 7441 ttgattgtaa cgaggattgt ggaacttctg ggacgcaggg ggtgggaagc cctcaaatat
 7501 tggtggaatc tcctacaata ttggagtcag gagctaaaga atagtgctgt tagcttgctc
 7561 aatgccacag ccatagcagt agctgagggg acagataggg ttatagaagt agtacaagga
 7621 gcttgtagag ctattcgcca catacctaga agaataagac agggcttgga aaggattttg
 7681 ctataagctc gaaacaaccg gtacctctag aactatagct agcagatctt tttccctctg
 7741 ccaaaaatta tggggacatc atgaagcccc ttgagcatct gacttctggc taataaagga
 7801 aatttatttt cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat
 7861 atgggagggc aaatcattta aaacatcaga atgagtattt ggtttagagt ttggcaacat
 7921 atgccatatg ctggctgcca tgaacaaagg tggctataaa gaggtcatca gtatatgaaa
 7981 cagccccctg ctgtccattc cttattccat agaaaagcct tgacttgagg ttagattttt
 8041 tttatatttt gttttgtgtt atttttttct ttaacatccc taaaattttc cttacatgtt
 8101 ttactagcca gatttttcct cctctcctga ctactcccag tcatagctgt ccctcttctc
 8161 ttatgaagat ccctcgacct gcagcccaag cttggcgtaa tcatggtcat agctgtttcc
 8221 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
 8281 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
 8341 cgctttccag tcgggaaacc tgtcgtgcca gcggatccgc atctcaatta gtcagcaacc
 8401 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
 8461 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
 8521 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagcta
 8581 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
 8641 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
 8701 atcatgtctg gatccgctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
 8761 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
 8821 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
 8881 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
 8941 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
 9001 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
 9061 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
 9121 cccttcggga agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta
 9181 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
 9241 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
 9301 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
 9361 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
 9421 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
 9481 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
 9541 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
 9601 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
 9661 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
 9721 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
 9781 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
 9841 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
 9901 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
 9961 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
10021 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
10081 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
10141 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
10201 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
10261 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
10321 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
10381 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
10441 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
10501 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
10561 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
10621 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
10681 atttccccga aaagtgccac ctg
//