PARD6B Protein & Antibody, Cell Lysate蛋白/抗体/细胞裂解液-BioVector NTCC基因蛋白抗体保藏中心

PARD6B蛋白/抗体/细胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因编号]: BC060847,BC060847.1,CB959958,CB959958.1 [Species物种来源]: Homo sapiens 【 PARD6B蛋白产品信息】 [Protein Construction蛋白构建]: A DNA sequence encoding the Target Protein was expressed. [Tag标签]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子种属]: Homo sapiens [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping运输说明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ PARD6B Protein QC Testing蛋白质控检测] Purity纯度: > 97 % as determined by SDS-PAGE Endotoxin内毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability稳定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation缓冲液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指导] [Storage储存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution复溶条件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 PARD6B Antibody Product Information抗体产品信息】 [Immunogen免疫原]: Recombinant Homo sapiens PARD6B protein [Ig Type Ig类型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation缓冲液]: 0.2 μm filtered solution in PBS [Preparation抗体制备方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 PARD6B Antibody Usage Guide抗体使用指导】 [Specificity特异性]: Homo sapiens PARD6B [Application推荐应用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage储存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 PARD6B Transfected / Overexpression Cell Lysate Product Information细胞裂解液产品信息】 [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description产品介绍]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 PARD6B Transfected / Overexpression Cell Lysate Usage Guide细胞裂解液使用指导】 [Preparation Method制备方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解缓冲液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing质控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability稳定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建议]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer储存缓冲液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction储存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes应用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.

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CAF1,CNOT7,HCAF-1 Protein & Antibody, Cell Lysate蛋白/抗体/细胞裂解液-BioVector NTCC基因蛋白抗体保藏中心

CAF1,CNOT7,HCAF-1蛋白/抗体/细胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因编号]: BC060852,BC060852.1,BI462880,BI462880.1 [Species物种来源]: Homo sapiens 【 CAF1,CNOT7,HCAF-1蛋白产品信息】 [Protein Construction蛋白构建]: A DNA sequence encoding the Target Protein was expressed. [Tag标签]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子种属]: Homo sapiens [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping运输说明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ CAF1,CNOT7,HCAF-1 Protein QC Testing蛋白质控检测] Purity纯度: > 97 % as determined by SDS-PAGE Endotoxin内毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability稳定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation缓冲液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指导] [Storage储存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution复溶条件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 CAF1,CNOT7,HCAF-1 Antibody Product Information抗体产品信息】 [Immunogen免疫原]: Recombinant Homo sapiens CAF1,CNOT7,HCAF-1 protein [Ig Type Ig类型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation缓冲液]: 0.2 μm filtered solution in PBS [Preparation抗体制备方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 CAF1,CNOT7,HCAF-1 Antibody Usage Guide抗体使用指导】 [Specificity特异性]: Homo sapiens CAF1,CNOT7,HCAF-1 [Application推荐应用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage储存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 CAF1,CNOT7,HCAF-1 Transfected / Overexpression Cell Lysate Product Information细胞裂解液产品信息】 [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description产品介绍]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 CAF1,CNOT7,HCAF-1 Transfected / Overexpression Cell Lysate Usage Guide细胞裂解液使用指导】 [Preparation Method制备方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解缓冲液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing质控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability稳定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建议]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer储存缓冲液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction储存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes应用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.

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SMEK2,FLJ31474,KIAA1387 Protein & Antibody, Cell Lysate蛋白/抗体/细胞裂解液-BioVector NTCC基因蛋白抗体保藏中心

SMEK2,FLJ31474,KIAA1387蛋白/抗体/细胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因编号]: BC060855,BC060855.1,BI546114,BI546114.1 [Species物种来源]: Homo sapiens 【 SMEK2,FLJ31474,KIAA1387蛋白产品信息】 [Protein Construction蛋白构建]: A DNA sequence encoding the Target Protein was expressed. [Tag标签]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子种属]: Homo sapiens [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping运输说明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ SMEK2,FLJ31474,KIAA1387 Protein QC Testing蛋白质控检测] Purity纯度: > 97 % as determined by SDS-PAGE Endotoxin内毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability稳定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation缓冲液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指导] [Storage储存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution复溶条件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 SMEK2,FLJ31474,KIAA1387 Antibody Product Information抗体产品信息】 [Immunogen免疫原]: Recombinant Homo sapiens SMEK2,FLJ31474,KIAA1387 protein [Ig Type Ig类型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation缓冲液]: 0.2 μm filtered solution in PBS [Preparation抗体制备方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 SMEK2,FLJ31474,KIAA1387 Antibody Usage Guide抗体使用指导】 [Specificity特异性]: Homo sapiens SMEK2,FLJ31474,KIAA1387 [Application推荐应用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage储存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 SMEK2,FLJ31474,KIAA1387 Transfected / Overexpression Cell Lysate Product Information细胞裂解液产品信息】 [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description产品介绍]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 SMEK2,FLJ31474,KIAA1387 Transfected / Overexpression Cell Lysate Usage Guide细胞裂解液使用指导】 [Preparation Method制备方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解缓冲液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing质控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability稳定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建议]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer储存缓冲液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction储存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes应用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.

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BCD,KLKB1,CYP4V2,CYP4AH1,MGC43534 Protein & Antibody, Cell Lysate蛋白/抗体/细胞裂解液-BioVector NTCC基因蛋白抗体保藏中心

BCD,KLKB1,CYP4V2,CYP4AH1,MGC43534蛋白/抗体/细胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因编号]: BC060857,BC060857.1,CB997176,CB997176.1 [Species物种来源]: Homo sapiens 【 BCD,KLKB1,CYP4V2,CYP4AH1,MGC43534蛋白产品信息】 [Protein Construction蛋白构建]: A DNA sequence encoding the Target Protein was expressed. [Tag标签]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子种属]: Homo sapiens [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping运输说明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ BCD,KLKB1,CYP4V2,CYP4AH1,MGC43534 Protein QC Testing蛋白质控检测] Purity纯度: > 97 % as determined by SDS-PAGE Endotoxin内毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability稳定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation缓冲液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指导] [Storage储存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution复溶条件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 BCD,KLKB1,CYP4V2,CYP4AH1,MGC43534 Antibody Product Information抗体产品信息】 [Immunogen免疫原]: Recombinant Homo sapiens BCD,KLKB1,CYP4V2,CYP4AH1,MGC43534 protein [Ig Type Ig类型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation缓冲液]: 0.2 μm filtered solution in PBS [Preparation抗体制备方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 BCD,KLKB1,CYP4V2,CYP4AH1,MGC43534 Antibody Usage Guide抗体使用指导】 [Specificity特异性]: Homo sapiens BCD,KLKB1,CYP4V2,CYP4AH1,MGC43534 [Application推荐应用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage储存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 BCD,KLKB1,CYP4V2,CYP4AH1,MGC43534 Transfected / Overexpression Cell Lysate Product Information细胞裂解液产品信息】 [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description产品介绍]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 BCD,KLKB1,CYP4V2,CYP4AH1,MGC43534 Transfected / Overexpression Cell Lysate Usage Guide细胞裂解液使用指导】 [Preparation Method制备方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解缓冲液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing质控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability稳定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建议]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer储存缓冲液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction储存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes应用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.

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FAM100B Protein & Antibody, Cell Lysate蛋白/抗体/细胞裂解液-BioVector NTCC基因蛋白抗体保藏中心

FAM100B蛋白/抗体/细胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因编号]: BC060859,BC060859.1,CB991653,CB991653.1 [Species物种来源]: Homo sapiens 【 FAM100B蛋白产品信息】 [Protein Construction蛋白构建]: A DNA sequence encoding the Target Protein was expressed. [Tag标签]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子种属]: Homo sapiens [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping运输说明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ FAM100B Protein QC Testing蛋白质控检测] Purity纯度: > 97 % as determined by SDS-PAGE Endotoxin内毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability稳定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation缓冲液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指导] [Storage储存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution复溶条件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 FAM100B Antibody Product Information抗体产品信息】 [Immunogen免疫原]: Recombinant Homo sapiens FAM100B protein [Ig Type Ig类型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation缓冲液]: 0.2 μm filtered solution in PBS [Preparation抗体制备方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 FAM100B Antibody Usage Guide抗体使用指导】 [Specificity特异性]: Homo sapiens FAM100B [Application推荐应用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage储存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 FAM100B Transfected / Overexpression Cell Lysate Product Information细胞裂解液产品信息】 [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description产品介绍]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 FAM100B Transfected / Overexpression Cell Lysate Usage Guide细胞裂解液使用指导】 [Preparation Method制备方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解缓冲液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing质控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability稳定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建议]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer储存缓冲液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction储存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes应用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.

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AGO61,C3ORF39,FLJ14566 Protein & Antibody, Cell Lysate蛋白/抗体/细胞裂解液-BioVector NTCC基因蛋白抗体保藏中心

AGO61,C3ORF39,FLJ14566蛋白/抗体/细胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因编号]: BC060861,BC060861.1,CB994328,CB994328.1 [Species物种来源]: Homo sapiens 【 AGO61,C3ORF39,FLJ14566蛋白产品信息】 [Protein Construction蛋白构建]: A DNA sequence encoding the Target Protein was expressed. [Tag标签]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子种属]: Homo sapiens [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping运输说明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ AGO61,C3ORF39,FLJ14566 Protein QC Testing蛋白质控检测] Purity纯度: > 97 % as determined by SDS-PAGE Endotoxin内毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability稳定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation缓冲液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指导] [Storage储存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution复溶条件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 AGO61,C3ORF39,FLJ14566 Antibody Product Information抗体产品信息】 [Immunogen免疫原]: Recombinant Homo sapiens AGO61,C3ORF39,FLJ14566 protein [Ig Type Ig类型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation缓冲液]: 0.2 μm filtered solution in PBS [Preparation抗体制备方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 AGO61,C3ORF39,FLJ14566 Antibody Usage Guide抗体使用指导】 [Specificity特异性]: Homo sapiens AGO61,C3ORF39,FLJ14566 [Application推荐应用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage储存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 AGO61,C3ORF39,FLJ14566 Transfected / Overexpression Cell Lysate Product Information细胞裂解液产品信息】 [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description产品介绍]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 AGO61,C3ORF39,FLJ14566 Transfected / Overexpression Cell Lysate Usage Guide细胞裂解液使用指导】 [Preparation Method制备方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解缓冲液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing质控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability稳定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建议]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer储存缓冲液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction储存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes应用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.

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CSNK2A1,GALNT15,GALNT18,GALNTL4,MGC71806,GALNAC-T15 Protein & Antibody, Cell Lysate蛋白/抗体/细胞裂解液-BioVector NTCC基因蛋白抗体保藏中心

CSNK2A1,GALNT15,GALNT18,GALNTL4,MGC71806,GALNAC-T15蛋白/抗体/细胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因编号]: BC060864,BC060864.1,CD109488,CD109488.1 [Species物种来源]: Homo sapiens 【 CSNK2A1,GALNT15,GALNT18,GALNTL4,MGC71806,GALNAC-T15蛋白产品信息】 [Protein Construction蛋白构建]: A DNA sequence encoding the Target Protein was expressed. [Tag标签]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子种属]: Homo sapiens [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping运输说明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ CSNK2A1,GALNT15,GALNT18,GALNTL4,MGC71806,GALNAC-T15 Protein QC Testing蛋白质控检测] Purity纯度: > 97 % as determined by SDS-PAGE Endotoxin内毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability稳定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation缓冲液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指导] [Storage储存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution复溶条件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 CSNK2A1,GALNT15,GALNT18,GALNTL4,MGC71806,GALNAC-T15 Antibody Product Information抗体产品信息】 [Immunogen免疫原]: Recombinant Homo sapiens CSNK2A1,GALNT15,GALNT18,GALNTL4,MGC71806,GALNAC-T15 protein [Ig Type Ig类型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation缓冲液]: 0.2 μm filtered solution in PBS [Preparation抗体制备方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 CSNK2A1,GALNT15,GALNT18,GALNTL4,MGC71806,GALNAC-T15 Antibody Usage Guide抗体使用指导】 [Specificity特异性]: Homo sapiens CSNK2A1,GALNT15,GALNT18,GALNTL4,MGC71806,GALNAC-T15 [Application推荐应用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage储存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 CSNK2A1,GALNT15,GALNT18,GALNTL4,MGC71806,GALNAC-T15 Transfected / Overexpression Cell Lysate Product Information细胞裂解液产品信息】 [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description产品介绍]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 CSNK2A1,GALNT15,GALNT18,GALNTL4,MGC71806,GALNAC-T15 Transfected / Overexpression Cell Lysate Usage Guide细胞裂解液使用指导】 [Preparation Method制备方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解缓冲液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing质控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability稳定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建议]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer储存缓冲液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction储存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes应用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.

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ANKRD38,FLJ10884,KIAA0172,DJ1078M7.1,RP5-1155K23.5 Protein & Antibody, Cell Lysate蛋白/抗体/细胞裂解液-BioVector NTCC基因蛋白抗体保藏中心

ANKRD38,FLJ10884,KIAA0172,DJ1078M7.1,RP5-1155K23.5蛋白/抗体/细胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因编号]: BC060866,BC060866.1,CB960938,CB960938.1 [Species物种来源]: Homo sapiens 【 ANKRD38,FLJ10884,KIAA0172,DJ1078M7.1,RP5-1155K23.5蛋白产品信息】 [Protein Construction蛋白构建]: A DNA sequence encoding the Target Protein was expressed. [Tag标签]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子种属]: Homo sapiens [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping运输说明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ ANKRD38,FLJ10884,KIAA0172,DJ1078M7.1,RP5-1155K23.5 Protein QC Testing蛋白质控检测] Purity纯度: > 97 % as determined by SDS-PAGE Endotoxin内毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability稳定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation缓冲液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指导] [Storage储存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution复溶条件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 ANKRD38,FLJ10884,KIAA0172,DJ1078M7.1,RP5-1155K23.5 Antibody Product Information抗体产品信息】 [Immunogen免疫原]: Recombinant Homo sapiens ANKRD38,FLJ10884,KIAA0172,DJ1078M7.1,RP5-1155K23.5 protein [Ig Type Ig类型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation缓冲液]: 0.2 μm filtered solution in PBS [Preparation抗体制备方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 ANKRD38,FLJ10884,KIAA0172,DJ1078M7.1,RP5-1155K23.5 Antibody Usage Guide抗体使用指导】 [Specificity特异性]: Homo sapiens ANKRD38,FLJ10884,KIAA0172,DJ1078M7.1,RP5-1155K23.5 [Application推荐应用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage储存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 ANKRD38,FLJ10884,KIAA0172,DJ1078M7.1,RP5-1155K23.5 Transfected / Overexpression Cell Lysate Product Information细胞裂解液产品信息】 [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description产品介绍]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 ANKRD38,FLJ10884,KIAA0172,DJ1078M7.1,RP5-1155K23.5 Transfected / Overexpression Cell Lysate Usage Guide细胞裂解液使用指导】 [Preparation Method制备方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解缓冲液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing质控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability稳定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建议]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer储存缓冲液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction储存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes应用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.

 Biovector

CV2,CV-2,BMPER,CRIM3 Protein & Antibody, Cell Lysate蛋白/抗体/细胞裂解液-BioVector NTCC基因蛋白抗体保藏中心

CV2,CV-2,BMPER,CRIM3蛋白/抗体/细胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因编号]: BC060868,BC060868.1,CB990561,CB990561.1 [Species物种来源]: Homo sapiens 【 CV2,CV-2,BMPER,CRIM3蛋白产品信息】 [Protein Construction蛋白构建]: A DNA sequence encoding the Target Protein was expressed. [Tag标签]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子种属]: Homo sapiens [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping运输说明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ CV2,CV-2,BMPER,CRIM3 Protein QC Testing蛋白质控检测] Purity纯度: > 97 % as determined by SDS-PAGE Endotoxin内毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability稳定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation缓冲液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指导] [Storage储存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution复溶条件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 CV2,CV-2,BMPER,CRIM3 Antibody Product Information抗体产品信息】 [Immunogen免疫原]: Recombinant Homo sapiens CV2,CV-2,BMPER,CRIM3 protein [Ig Type Ig类型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation缓冲液]: 0.2 μm filtered solution in PBS [Preparation抗体制备方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 CV2,CV-2,BMPER,CRIM3 Antibody Usage Guide抗体使用指导】 [Specificity特异性]: Homo sapiens CV2,CV-2,BMPER,CRIM3 [Application推荐应用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage储存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 CV2,CV-2,BMPER,CRIM3 Transfected / Overexpression Cell Lysate Product Information细胞裂解液产品信息】 [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description产品介绍]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 CV2,CV-2,BMPER,CRIM3 Transfected / Overexpression Cell Lysate Usage Guide细胞裂解液使用指导】 [Preparation Method制备方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解缓冲液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing质控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability稳定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建议]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer储存缓冲液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction储存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes应用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.

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FLJ34104,KIAA1946 Protein & Antibody, Cell Lysate蛋白/抗体/细胞裂解液-BioVector NTCC基因蛋白抗体保藏中心

FLJ34104,KIAA1946蛋白/抗体/细胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因编号]: BC060872,BC060872.1,CB989566,CB989566.1 [Species物种来源]: Homo sapiens 【 FLJ34104,KIAA1946蛋白产品信息】 [Protein Construction蛋白构建]: A DNA sequence encoding the Target Protein was expressed. [Tag标签]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子种属]: Homo sapiens [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping运输说明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ FLJ34104,KIAA1946 Protein QC Testing蛋白质控检测] Purity纯度: > 97 % as determined by SDS-PAGE Endotoxin内毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability稳定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation缓冲液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指导] [Storage储存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution复溶条件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 FLJ34104,KIAA1946 Antibody Product Information抗体产品信息】 [Immunogen免疫原]: Recombinant Homo sapiens FLJ34104,KIAA1946 protein [Ig Type Ig类型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation缓冲液]: 0.2 μm filtered solution in PBS [Preparation抗体制备方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 FLJ34104,KIAA1946 Antibody Usage Guide抗体使用指导】 [Specificity特异性]: Homo sapiens FLJ34104,KIAA1946 [Application推荐应用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage储存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 FLJ34104,KIAA1946 Transfected / Overexpression Cell Lysate Product Information细胞裂解液产品信息】 [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description产品介绍]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 FLJ34104,KIAA1946 Transfected / Overexpression Cell Lysate Usage Guide细胞裂解液使用指导】 [Preparation Method制备方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解缓冲液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing质控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability稳定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建议]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer储存缓冲液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction储存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes应用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.

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