DAN-G BioVector®人胰腺癌细胞系human pancreatic carcinom Cell Line-广泛用于胰腺癌的研究

Product Name / 产品名称:
BioVector® DAN-G Cell Line人胰腺癌细胞系-广泛用于胰腺癌的研究

Description / 描述:
The BioVector® DAN-G cell line is a well-characterized human pancreatic carcinoma cell line derived from a 68-year-old female patient -2. It is extensively utilized in research focused on pancreatic cancer, particularly in studies pertaining to tumorigenesis, metastasis, and chemotherapy resistance -2. The genetic profile of DAN-G includes mutations in key oncogenes and tumor suppressor genes characteristic of pancreatic adenocarcinomas, making it a valuable model for understanding molecular mechanisms and testing therapeutic strategies -2.
BioVector® DAN-G 细胞系是一种特性明确的人胰腺癌细胞系，来源于一位68岁的女性患者 -2。该细胞系广泛用于胰腺癌的研究，特别是在肿瘤发生、转移和化疗耐药性方面 -2。DAN-G的遗传特征包括关键癌基因和抑癌基因的突变，这些是胰腺腺癌的典型特征，使其成为理解分子机制和测试治疗策略的宝贵模型 -2。

Species / 物种:
Human / 人

Tissue Origin / 组织来源:
Pancreas / 胰腺

Disease / 疾病:
Pancreatic Carcinoma / Adenocarcinoma -1-2
胰腺癌 / 腺癌

Synonyms / 同义词:
Dan-G, DanG, DANG -2

Morphology / 形态:
Epithelial-like -2 / 上皮样

Growth Properties / 生长特性:
Adherent -2 / 贴壁

Gender / 性别:
Female -2 / 女性

Age / 年龄:
68 years -2 / 68岁

Key Applications / 主要应用:

• Pancreatic cancer research / 胰腺癌研究

• Tumorigenesis and metastasis studies / 肿瘤发生和转移研究

• Chemotherapy resistance research / 化疗耐药性研究

• Drug screening and efficacy testing / 药物筛选和疗效测试

• Xenograft tumor models in immunocompromised mice -2 / 免疫缺陷小鼠异种移植肿瘤模型

Key Characteristics / 关键特征:

Culture Conditions / 培养条件:

Subculture Protocol / 传代方案:

1. Remove old medium and wash with BioVector® PBS (without Ca²⁺/Mg²⁺) / 弃去旧培养基，用 BioVector® PBS（不含钙镁）清洗

2. Cover cells completely with BioVector® Accutase Solution (1-2 ml for T25 flask) / 用 BioVector® Accutase 消化液 完全覆盖细胞（T25瓶用1-2 ml）

3. Incubate at room temperature for 8-10 minutes to detach cells -2 / 室温孵育8-10分钟使细胞脱落

4. Gently mix with 10 ml medium to resuspend, centrifuge at 300×g for 3 minutes -2 / 轻轻加入10 ml培养基重悬，300×g离心3分钟

5. Discard supernatant, resuspend in fresh medium, and transfer to new flasks / 弃上清，用新鲜培养基重悬，转移至新培养瓶

Post-Thaw Recovery / 复苏后培养:
Plate cells at 5 × 10⁴ cells/cm² and allow at least 24 hours for recovery and adhesion -2.
按5 × 10⁴细胞/cm²接种，至少培养24小时使细胞复苏和贴壁 -2。

Freeze Medium / 冻存液:
BioVector® Cryopreservation Medium (complete growth medium + 10% DMSO) or BioVector® CM-1 Cryopreservation Medium for enhanced recovery -2.
BioVector® 细胞冻存液（完全培养基 + 10% DMSO）或 BioVector® CM-1 细胞冻存液，以获得更佳复苏效果。

Quality Control / 质量控制:

• Sterility / 无菌检测: Negative for bacterial and fungal contamination -2 / 细菌和真菌检测阴性

• Mycoplasma / 支原体检测: Negative by PCR-based and luminescence-based assays -2 / PCR和发光法检测阴性

Storage / 存储:

• Cells are shipped on dry ice. Upon receipt, immediately transfer to liquid nitrogen vapor phase for long-term storage (below -150°C) -2. Short-term storage at -80°C is acceptable only as an interim step -2.

• 细胞干冰运输。收到后，立即转移至液氮气相长期保存（-150°C以下）。-80°C仅可短期临时保存。

Citation / 引用:
When using this cell line, please cite as: DAN-G (BioVector® catalog number 300162) -2
使用此细胞系时，请引用：DAN-G (BioVector® 目录号 300162)

Related Products / 相关产品:

Important Note / 重要提示:

• The DAN-G cell line carries a homozygous Kras G12V mutation and is P53 negative, making it representative of aggressive pancreatic ductal adenocarcinoma -2.

• DAN-G细胞系携带纯合Kras G12V突变且P53阴性，使其具有侵袭性胰腺导管腺癌的代表性。

• These cells are tumorigenic in nude mice, providing an in vivo model for efficacy evaluation of anticancer drugs -2.

• 该细胞在裸鼠中具有致瘤性，为抗癌药物的体内疗效评估提供了模型。

• SNP array data from the Cancer Cell Line Encyclopedia (CCLE) is available for this cell line (NCBI GEO: GSM888040) -1.

• 该细胞系的SNP芯片数据可在癌症细胞系百科全书（CCLE）中获取（NCBI GEO: GSM888040）

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