ICC3 BioVector®人肝外胆管癌细胞株human Extrahepatic Cholangiocarcinoma cell line

• 细胞名称: ICC3

• DSMZ 编号: ACC 995

• 别名: MGH-ECC4, ECC4 (Cellosaurus ID: CVCL_A1ZB)

• 物种: 人类 (Homo sapiens)

• 性别: 女性，63岁

• 疾病类型: 肝外胆管癌 (Extrahepatic Cholangiocarcinoma, ECC)

• 建立背景: 2016年建立自原发肿瘤的患者来源异种移植模型 (PDX)。

• 生物安全等级: 1 (在进行基因工程操作时，建议提升至 BSL-2)

• 驱动突变:

  • KRAS p.G12D: 常见的致癌驱动突变，通常导致 MAPK 和 PI3K 通路的持续激活。

  • CTNNB1 p.S33P: 涉及 Wnt/β-catenin 通路的激活。

  
  

• STR 鉴定:

  • Amelogenin: X

  • CSF1PO: 9, 10

  • D13S317: 12, 14

  • D16S539: 11, 12

  • D5S818: 10, 12。

  
  

• 其他特征: 端粒酶活性阴性（TRAP检测）；多种常见病毒（HIV, HBV, HCV, EBV等）检测均为阴性。

• 形态学: 类上皮细胞，贴壁呈单层生长，伴有少量悬浮细胞。

• 倍增时间: 约 30-40 小时。

• 关键操作:

  • 不可丢弃悬浮细胞: 更换培养基时需保留上清液中的细胞，因为它们具有活力。

  • 耗材建议: 细胞在重复使用的培养瓶中贴壁较差，建议使用一次性高品质 T75 培养瓶。

  
  

• 培养基: 90% RPMI 1640 + 10% 热灭活胎牛血清 (h.i. FBS)。

BioVector® ICC3 Cell Line

• Cell Line Name: ICC3

• DSMZ No.:ACC 995

• Aliases: MGH-ECC4, ECC4 (Cellosaurus ID: CVCL_A1ZB)

• Species: Human (Homo sapiens)

• Sex/Age: Female, 63 years old.

• Disease:Extrahepatic Cholangiocarcinoma (ECC).

• Establishment: Established in 2016 from a Patient-Derived Xenograft (PDX) model of the primary tumor.

• Biosafety Level: 1 (Note: Handle at BSL-2 if used in genetic engineering projects).

• Key Mutations:

  • KRAS p.G12D: A potent oncogenic driver affecting cellular proliferation pathways like MAPK/ERK.

  • CTNNB1 p.S33P: A mutation in the β-catenin gene associated with Wnt pathway activation.

  
  

• STR Authentication: Profile includes markers such as D13S317 (12, 14), D7S820 (7, 11), and TH01 (6, 9.3).

• Stability: Negative for telomerase activity via TRAP assay; verified authentic against reference databases.

• Morphology: Epithelial-like, growing as an adherent monolayer with a significant population of viable cells in suspension.

• Doubling Time: Approximately 30-40 hours.

• Yield: Approximately 9 x 10⁶ cells per T75 flask at harvest.

• Technical Tips:

  • Supernatant Management: It is vital not to discard cells in the supernatant during media changes as they are part of the active culture.

  • Adhesion Issues: Cells exhibit poor attachment in re-used flasks; fresh plasticware is recommended for consistent growth.

  
  

• Medium: 90% RPMI 1640 + 10% heat-inactivated FBS.

Supplier生产厂家:

BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心

BioVector NTCC Inc.

TEL: 400-800-2947

E-mail: biovector@163.com

工作微信/QQ同号: 1843439339

http://www.biovector.net