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ackA E.coli mutant大肠杆菌基因缺失菌株-BioVector NTCC保藏中心
Gene Symbol突变基因名称:ackA
Mnemonic:acetate kinase
Synonyms别称:
Gene product基因产物:acetate kinase
Selectable Phenotype筛选表型:grow on media containing 25 mM pyruvate or proline as sole carbon sources despite the presence of 10 or 50 mM sodium fluoroacetate
Culture Condition培养条件:defined liquid media containing 50 mM-sodium/potassium phosphate buffer pH 7.2, 50 mM-ammonium chloride, essential salts (4 micro-g of CaCl2.6H20; 8 mg of MgSO4.7H2O; 0.4 mig of MnSO4.4H2O; 0.4 mg FeSO4.7H2O per 100 ml of medium) and carbon source at 25 mM
Other Phenotypes其他表型:•grow poorly on acetate (25 mM) as sole carbon source
Notes:•in ackA- pta-:grow poorly on high concentrations(more than 25 mM) (J. Bacteriol. 1995. 177: 2878-2886)
•in acs- ackA- pta-unable to grow on acetate at any concentration tested (J. Bacteriol. 1995. 177: 2878-2886)
Reference参考文献:Brown, T. D. K. et al.
J. Gen. Microbiol. 1977. 102: 327-336
Supplier:
BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
电话:+86-010-53513060
网址:www.biovector.net
Gene Symbol突变基因名称:ackA
Mnemonic:acetate kinase
Synonyms别称:
Gene product基因产物:acetate kinase
Selectable Phenotype筛选表型:grow on media containing 25 mM pyruvate or proline as sole carbon sources despite the presence of 10 or 50 mM sodium fluoroacetate
Culture Condition培养条件:defined liquid media containing 50 mM-sodium/potassium phosphate buffer pH 7.2, 50 mM-ammonium chloride, essential salts (4 micro-g of CaCl2.6H20; 8 mg of MgSO4.7H2O; 0.4 mig of MnSO4.4H2O; 0.4 mg FeSO4.7H2O per 100 ml of medium) and carbon source at 25 mM
Other Phenotypes其他表型:•grow poorly on acetate (25 mM) as sole carbon source
Notes:•in ackA- pta-:grow poorly on high concentrations(more than 25 mM) (J. Bacteriol. 1995. 177: 2878-2886)
•in acs- ackA- pta-unable to grow on acetate at any concentration tested (J. Bacteriol. 1995. 177: 2878-2886)
Reference参考文献:Brown, T. D. K. et al.
J. Gen. Microbiol. 1977. 102: 327-336
Supplier:
BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
电话:+86-010-53513060
网址:www.biovector.net
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