BHK-21 cell line细胞株-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心

Designations:  BHK-21 [C-13]

Depositors:      I Macpherson

Biosafety Level:        1

Shipped:   frozen

Medium & Serum:   See Propagation

Growth Properties: adherent

Organism:        Mesocricetusauratus

Morphology:    fibroblast

Source:     Organ:kidney

Disease: normal

Cell Type: fibroblast

Isolation: Isolationdate: March, 1961            

Applications:    testing

transfection host              

Virus Susceptibility: Human adenovirus 25

Reovirus 3

Vesicular stomatitis virus

Human poliovirus 2          

Cytogenetic Analysis:      Chromosome Frequency Distribution 50Cells: 2n = 44. This is a pseudodiploid line with the tetraploidy occurring at4%. The karyotype is 44,XY,-6,-15,6q+,15q+ in a majority of cells analyzed. Themarkers 6q+ and 15q+ occurred in most cells. An occasional monosomic ortrisomic condition for a normal chromosome was also detected.          

Age: 1day old newborn            

HeLa Markers:         N                

Comments:      Theparent line of BHK-21(C-13) was derived from the kidneys of five unsexed,1-day-old hamsters in March, 1961, by I.A. Macpherson and M.G.P. Stoker.

Following 84 days of continuouscultivation, interrupted only by an 8-day preservation by freezing, clone 13was initiated by single-cell isolation.

This line has been used as a host fortransformation with expression vectors containing selectable and amplifiablemarker DNAs (e.g., Factor VIII).

The World Organization for Animal Health(OIE) uses the cells for routine diagnosis of rabies. (see:http://www.oie.int/Eng/Normes/Mmanual/A_00044.htm).          

Propagation:    Eagle's Minimum Essential Medium, Catalog No. 30-2003. To makethe complete growth medium, add the following components to the base medium:fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C                

Subculturing:   Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTAsolution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTAsolution. Allow the flask to sit at room temperature (or at 37C) until thecells detach. Add fresh culture medium, aspirate and dispense into new cultureflasks.

Subcultivation Ratio: A subcultivationratio of 1:2 to 1:10 is recommended

Medium Renewal: 1 to 2 times per week                

Preservation:   Freeze medium: Complete growth medium 95%; DMSO, 5%

Storage temperature: liquid nitrogen vaporphase                  

Bioreactive Factors:         Growth Factors: T cell growth factor (TCGF)          

References:     2:Drayna D, et al. Genetic mapping and diagnosis of haemophilia A achievedthrough a BclI polymorphism in the factor VIII gene. Nature 314: 738-740, 1985.PubMed: 2986011

3: Kazazian HH Jr., et al. Restriction sitepolymorphism in the phosphoglycerate kinase gene on the X chromosome. Hum.Genet. 66: 217-219, 1984. PubMed: 6325324

21411: Macpherson I, Stoker M. Polyomatransformation of hamster cell clones--an investigation of genetic factorsaffecting cell competence. Virology 16: 147-151, 1962. PubMed: 14468055

21412: . . Virology 14: 359-370, 1961.

25963: Macpherson I. Characteristics of ahamster cell clone transformed by polyoma virus. J. Natl. Cancer Inst. 30:795-815, 1963.

27297: Deleersnyder V, et al. Formation ofnative hepatitis C virus glycoprotein complexes. J. Virol. 71: 697-704, 1997.PubMed: 8985401

32269: Yang TT, et al. Quantification ofgene expression with a secreted alkaline phosphatase reporter system.BioTechniques 23: 1110-1114, 1997. PubMed: 9421645

32473: Hussain MA, et al. POU domaintranscription factor brain 4 confers pancreatic alpha-cell-specific expressionof the proglucagon gene through interaction with a novel proximal promoter G1element. Mol. Cell. Biol. 17: 7186-7194, 1997. PubMed: 9372951

32475: You M, et al. ch-IAp1, a member ofthe inhibitor-of-apoptosis protein family, is a mediator of the antiapoptoticactivity of the v-Rel oncoprotein. Mol. Cell. Biol. 17: 7328-7341, 1997.PubMed: 9372964

32501: Jelachich ML, Lipton HL. Theiler'smurine encephalomyelitis virus kills restrictive but not permissive cells byapoptosis. J. Virol. 70: 6856-6861, 1996. PubMed: 8794327

32512: Schnell MJ, et al. The minimal conservedtranscription stop-start signal promotes stable expression of a foreign gene invesicular stomatitis virus. J. Virol. 70: 2318-2323, 1996. PubMed: 8642658

32524: Chang YE, et al. Properties of theprotein encoded by the UL32 open reading frame of herpes simplex virus 1. J.Virol. 70: 3938-3946, 1996. PubMed: 8648731

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